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sample_name:"mt-LAF4"
Trypanosoma brucei mitochondrial ribosome large subunit assembly intermediate
Release date: Dec. 2, 2020
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7aoi
Q-score: 0.486
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7aoi
Q-score: 0.486
Tobiasson V, Gahura O, Aibara S, Baradaran R, Zikova A, Amunts A
EMBO J (2021) 40 pp. e106292-e106292 [ DOI: doi:10.15252/embj.2020106292 Pubmed: 33576519 ]
Sample:
- Unk8 (1 kDa, Protein from Trypanosoma brucei)
- Ml52 (14 kDa, Protein from Trypanosoma brucei)
- Ml86 (19 kDa, Protein from Trypanosoma brucei)
- Ml74 (25 kDa, Protein from Trypanosoma brucei)
- Ml42 (15 kDa, Protein from Trypanosoma brucei)
- Gtp-binding protein, putative,mt-enga (60 kDa, Protein from Trypanosoma brucei)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Unk10 (1 kDa, Protein from Trypanosoma brucei)
- Mt-laf15_1 (21 kDa, Protein from Trypanosoma brucei)
- Ribosomal_l18e/l15p domain-containing protein (41 kDa, Protein from Trypanosoma brucei)
- Dead-box helicase, putative,mt-laf2 (76 kDa, Protein from Trypanosoma brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei)
- Ml87 (9 kDa, Protein from Trypanosoma brucei)
- Ml67 (89 kDa, Protein from Trypanosoma brucei)
- Bl32m (6 kDa, Protein from Trypanosoma brucei)
- Chaperone protein dnaj, putative,ml77,chaperone protein dnaj, putative (30 kDa, Protein from Trypanosoma brucei)
- Ml71 (49 kDa, Protein from Trypanosoma brucei)
- Mt-laf12 (70 kDa, Protein from Trypanosoma brucei)
- Magnesium ion (24 Da, Ligand)
- Ml85 (16 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l13 (20 kDa, Protein from Trypanosoma brucei)
- Unk1 (3 kDa, Protein from Trypanosoma brucei)
- Ml80 (25 kDa, Protein from Trypanosoma brucei)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Rna uridylyltransferase (17 kDa, Protein from Trypanosoma brucei)
- Ul24m (39 kDa, Protein from Trypanosoma brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei)
- Trypanosoma brucei mitochondrial ribosome large subunit assembly intermediate (2 MDa, Complex from Trypanosoma brucei)
- Ml100,ml100 (10 kDa, Protein from Trypanosoma brucei)
- Tetratricopeptide repeat (48 kDa, Protein from Trypanosoma brucei)
- Unk11 (3 kDa, Protein from Trypanosoma brucei)
- Bl20m (22 kDa, Protein from Trypanosoma brucei)
- Ml95 (16 kDa, Protein from Trypanosoma brucei)
- Mt-laf7,mt-laf7 (18 kDa, Protein from Trypanosoma brucei)
- Bl33m (17 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l14 (19 kDa, Protein from Trypanosoma brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei)
- Ml63 (17 kDa, Protein from Trypanosoma brucei)
- Ml70 (47 kDa, Protein from Trypanosoma brucei)
- Unk9 (2 kDa, Protein from Trypanosoma brucei)
- Ml94 (11 kDa, Protein from Trypanosoma brucei)
- Spou rrna methylase family (48 kDa, Protein from Trypanosoma brucei)
- Mitochondrial ribosomal protein l51 / s25 / ci-b8 domain containing protein (22 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l22p/l17e (31 kDa, Protein from Trypanosoma brucei)
- Ml101 (21 kDa, Protein from Trypanosoma brucei)
- Mt-laf14 (78 kDa, Protein from Trypanosoma brucei)
- 50s ribosomal protein l17 (30 kDa, Protein from Trypanosoma brucei)
- Ml53 (33 kDa, Protein from Trypanosoma brucei)
- Ml78 (31 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l23 (19 kDa, Protein from Trypanosoma brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l21 (20 kDa, Protein from Trypanosoma brucei)
- Unk6/mt-laf15_2 (9 kDa, Protein from Trypanosoma brucei)
- Mitochondrial rna binding complex 1 subunit (37 kDa, Protein from Trypanosoma brucei)
- Unk2 (657 Da, Protein from Trypanosoma brucei)
- Ribosomal_l9 domain-containing protein (25 kDa, Protein from Trypanosoma brucei)
- Ml81 (25 kDa, Protein from Trypanosoma brucei)
- Lipase (class 3) (18 kDa, Protein from Trypanosoma brucei)
- Ml99 (9 kDa, Protein from Trypanosoma brucei)
- Unk4 (586 Da, Protein from Trypanosoma brucei)
- Unk7 (1 kDa, Protein from Trypanosoma brucei)
- Ml98 (10 kDa, Protein from Trypanosoma brucei)
- Complex1-lyr-like (10 kDa, Protein from Trypanosoma brucei)
- Zinc ion (65 Da, Ligand)
- Mt-laf8 (58 kDa, Protein from Trypanosoma brucei)
- Ml41 (13 kDa, Protein from Trypanosoma brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Ribosomal protein l3 mitochondrial, putative (42 kDa, Protein from Trypanosoma brucei)
- Mt-lsu rrna (242 kDa, RNA from Trypanosoma brucei)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mt-laf19 (11 kDa, Protein from Trypanosoma brucei)
- Acyl carrier protein (9 kDa, Protein from Trypanosoma brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei)
- Ml68,ml68,ml68 (40 kDa, Protein from Trypanosoma brucei)
- Lim domain containing protein,ml90 (13 kDa, Protein from Trypanosoma brucei)
- Ml93 (16 kDa, Protein from Trypanosoma brucei)
- Unk3 (1 kDa, Protein from Trypanosoma brucei)
- Ml76 (18 kDa, Protein from Trypanosoma brucei)
- Ml49 (20 kDa, Protein from Trypanosoma brucei)
- Ul30m (17 kDa, Protein from Trypanosoma brucei)
- Ul29m (53 kDa, Protein from Trypanosoma brucei)
- Gtp-binding protein (33 kDa, Protein from Trypanosoma brucei)
- Unk5 (870 Da, Protein from Trypanosoma brucei)
- Pseudouridylate synthase, putative (42 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l4/l1 family (51 kDa, Protein from Trypanosoma brucei)
- Bl28m (25 kDa, Protein from Trypanosoma brucei)
- Ribosomal protein l11,ul11m (29 kDa, Protein from Trypanosoma brucei)
- Ml84 (23 kDa, Protein from Trypanosoma brucei)
- Ml64,ml64 (23 kDa, Protein from Trypanosoma brucei)
State A of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Release date: Oct. 14, 2020
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6yxx
Q-score: 0.389
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6yxx
Q-score: 0.389
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol Cell (2020) 79 pp. 629-644.e4 [ DOI: doi:10.1016/j.molcel.2020.06.030 Pubmed: 32679035 ]
Sample:
- State b of the trypanosoma brucei mitoribosomal large subunit assembly intermediate (Complex from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Ml95 (17 kDa, Protein from Trypanosoma brucei brucei)
- Ul29m (54 kDa, Protein from Trypanosoma brucei brucei)
- Ml52 (167 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf24 (49 kDa, Protein from Trypanosoma brucei brucei)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Mt-laf7 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ul23m (28 kDa, Protein from Trypanosoma brucei brucei)
- Mt-enga (65 kDa, Protein from Trypanosoma brucei brucei)
- Peptidyl-prolyl cis-trans isomerase (25 kDa, Protein from Trypanosoma brucei brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ml71 (50 kDa, Protein from Trypanosoma brucei brucei)
- Bl21m (20 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Chaperone protein dnaj, putative (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml76 (39 kDa, Protein from Trypanosoma brucei brucei)
- Duf4379 domain-containing protein (63 kDa, Protein from Trypanosoma brucei brucei)
- Ml74 (39 kDa, Protein from Trypanosoma brucei brucei)
- Ul24m (43 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei brucei)
- Spou_methylase domain-containing protein (40 kDa, Protein from Trypanosoma brucei brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l4/l1 family, putative (53 kDa, Protein from Trypanosoma brucei brucei)
- Ul30m (25 kDa, Protein from Trypanosoma brucei brucei)
- 50s ribosomal protein l17, putative (35 kDa, Protein from Trypanosoma brucei brucei)
- Ml98 (12 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l3 mitochondrial, putative (55 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf27 (11 kDa, Protein from Trypanosoma brucei brucei)
- Ul22m (32 kDa, Protein from Trypanosoma brucei brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei brucei)
- Unk (698 Da, Protein from Trypanosoma brucei brucei)
- Acyl carrier protein (16 kDa, Protein from Trypanosoma brucei brucei)
- Ml70 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ml75 (39 kDa, Protein from Trypanosoma brucei brucei)
- Bl20m (24 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Unk (1 kDa, Protein from Trypanosoma brucei brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Spou_methylase domain-containing protein (67 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal_l9 domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Ml81 (29 kDa, Protein from Trypanosoma brucei brucei)
- Ml49 (24 kDa, Protein from Trypanosoma brucei brucei)
- 50s ribosomal protein l13, putative (24 kDa, Protein from Trypanosoma brucei brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei brucei)
- Bl35m (21 kDa, Protein from Trypanosoma brucei brucei)
- Kripp9 (89 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf12 (77 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf25 (32 kDa, Protein from Trypanosoma brucei brucei)
- Sodium ion (22 Da, Ligand)
- Ml88 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l11, putative (40 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf15a (34 kDa, Protein from Trypanosoma brucei brucei)
- Ml63 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ml80 (34 kDa, Protein from Trypanosoma brucei brucei)
- Unk (6 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf29 (38 kDa, Protein from Trypanosoma brucei brucei)
- L51_s25_ci-b8 domain-containing protein (31 kDa, Protein from Trypanosoma brucei brucei)
- Unk (20 kDa, Protein from Trypanosoma brucei brucei)
- Ml84 (24 kDa, Protein from Trypanosoma brucei brucei)
- Water (18 Da, Ligand)
- Ribosomal_l18e/l15p domain-containing protein (43 kDa, Protein from Trypanosoma brucei brucei)
- Dead-box helicase, putative (85 kDa, Protein from Trypanosoma brucei brucei)
- Ml85 (22 kDa, Protein from Trypanosoma brucei brucei)
- 12s ribosomal rna (365 kDa, RNA from Trypanosoma brucei brucei)
- Ml53 (35 kDa, Protein from Trypanosoma brucei brucei)
- Tetratricopeptide repeat (49 kDa, Protein from Trypanosoma brucei brucei)
- Ml93 (18 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Mt-laf14 (82 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf23 (63 kDa, Protein from Trypanosoma brucei brucei)
- Unk (7 kDa, Protein from Trypanosoma brucei brucei)
- Ml67 (93 kDa, Protein from Trypanosoma brucei brucei)
- Kripp3 (58 kDa, Protein from Trypanosoma brucei brucei)
- Ml86 (22 kDa, Protein from Trypanosoma brucei brucei)
- S-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate (496 Da, Ligand)
- Gtp-binding protein, putative (50 kDa, Protein from Trypanosoma brucei brucei)
- Ml78 (35 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf21 (56 kDa, Protein from Trypanosoma brucei brucei)
- Ml41 (22 kDa, Protein from Trypanosoma brucei brucei)
- Bl28m (28 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Pseudouridylate synthase, putative (46 kDa, Protein from Trypanosoma brucei brucei)
- Ml42 (21 kDa, Protein from Trypanosoma brucei brucei)
- Ml64 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ml68 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ml94 (17 kDa, Protein from Trypanosoma brucei brucei)
- Bl32m (9 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf8 (68 kDa, Protein from Trypanosoma brucei brucei)
- Ml87 (22 kDa, Protein from Trypanosoma brucei brucei)
- Unk (869 Da, Protein from Trypanosoma brucei brucei)
- Mt-laf19 (12 kDa, Protein from Trypanosoma brucei brucei)
- Lipase (class 3) (58 kDa, Protein from Trypanosoma brucei brucei)
State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Release date: Oct. 14, 2020
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6yxy
Q-score: 0.508
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6yxy
Q-score: 0.508
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol Cell (2020) 79 pp. 629-644.e4 [ DOI: doi:10.1016/j.molcel.2020.06.030 Pubmed: 32679035 ]
Sample:
- State b of the trypanosoma brucei mitoribosomal large subunit assembly intermediate (Complex from Trypanosoma brucei brucei)
- Ml95 (17 kDa, Protein from Trypanosoma brucei brucei)
- Ul29m (54 kDa, Protein from Trypanosoma brucei brucei)
- Ml52 (167 kDa, Protein from Trypanosoma brucei brucei)
- Spermidine (145 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Unk (15 kDa, Protein from Trypanosoma brucei brucei)
- Unk (6 kDa, Protein from Trypanosoma brucei brucei)
- Unk (1 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf7 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ul23m (28 kDa, Protein from Trypanosoma brucei brucei)
- Mt-enga (65 kDa, Protein from Trypanosoma brucei brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ml71 (50 kDa, Protein from Trypanosoma brucei brucei)
- Ml101 (28 kDa, Protein from Trypanosoma brucei brucei)
- Bl21m (20 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Ul15m (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml90 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml76 (39 kDa, Protein from Trypanosoma brucei brucei)
- Ul14m (25 kDa, Protein from Trypanosoma brucei brucei)
- Ml74 (39 kDa, Protein from Trypanosoma brucei brucei)
- Bl9m (30 kDa, Protein from Trypanosoma brucei brucei)
- Ul24m (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml99 (12 kDa, Protein from Trypanosoma brucei brucei)
- L0r8f8 (13 kDa, Protein from Trypanosoma brucei brucei)
- Ml43 (31 kDa, Protein from Trypanosoma brucei brucei)
- Ul30m (25 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Ml98 (12 kDa, Protein from Trypanosoma brucei brucei)
- Ul11m (40 kDa, Protein from Trypanosoma brucei brucei)
- Ul22m (32 kDa, Protein from Trypanosoma brucei brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei brucei)
- Unk (698 Da, Protein from Trypanosoma brucei brucei)
- Ml70 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ml75 (39 kDa, Protein from Trypanosoma brucei brucei)
- 12s ribosomal rna (373 kDa, RNA from Trypanosoma brucei brucei)
- Bl20m (24 kDa, Protein from Trypanosoma brucei brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Mt-engb (71 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf18 (58 kDa, Protein from Trypanosoma brucei brucei)
- Mt-acp (16 kDa, Protein from Trypanosoma brucei brucei)
- Ml81 (29 kDa, Protein from Trypanosoma brucei brucei)
- Ml49 (24 kDa, Protein from Trypanosoma brucei brucei)
- Ul13m (24 kDa, Protein from Trypanosoma brucei brucei)
- Ml72 (49 kDa, Protein from Trypanosoma brucei brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei brucei)
- Bl35m (21 kDa, Protein from Trypanosoma brucei brucei)
- Ml83 (25 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf12 (77 kDa, Protein from Trypanosoma brucei brucei)
- Ml92 (20 kDa, Protein from Trypanosoma brucei brucei)
- Sodium ion (22 Da, Ligand)
- Ml88 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf15a (34 kDa, Protein from Trypanosoma brucei brucei)
- Ml63 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ml80 (34 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf6 (40 kDa, Protein from Trypanosoma brucei brucei)
- Ml84 (24 kDa, Protein from Trypanosoma brucei brucei)
- Water (18 Da, Ligand)
- Ml85 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml53 (35 kDa, Protein from Trypanosoma brucei brucei)
- Ml100 (10 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf5 (67 kDa, Protein from Trypanosoma brucei brucei)
- Ml93 (18 kDa, Protein from Trypanosoma brucei brucei)
- Mtg1 (50 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Mt-laf14 (82 kDa, Protein from Trypanosoma brucei brucei)
- Ml67 (93 kDa, Protein from Trypanosoma brucei brucei)
- Ml77 (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml86 (22 kDa, Protein from Trypanosoma brucei brucei)
- S-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate (496 Da, Ligand)
- Mt-laf2 (85 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf3 (46 kDa, Protein from Trypanosoma brucei brucei)
- Ul3m (55 kDa, Protein from Trypanosoma brucei brucei)
- Ul4m (53 kDa, Protein from Trypanosoma brucei brucei)
- Ml78 (35 kDa, Protein from Trypanosoma brucei brucei)
- Bl17m (35 kDa, Protein from Trypanosoma brucei brucei)
- Ml41 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf20 (6 kDa, Protein from Trypanosoma brucei brucei)
- Bl28m (28 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Ml42 (21 kDa, Protein from Trypanosoma brucei brucei)
- Ml64 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ml68 (62 kDa, Protein from Trypanosoma brucei brucei)
- Malsu1 (39 kDa, Protein from Trypanosoma brucei brucei)
- Ml94 (17 kDa, Protein from Trypanosoma brucei brucei)
- Bl32m (9 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf8 (68 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei brucei)
- Ml87 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf19 (12 kDa, Protein from Trypanosoma brucei brucei)